Assay chromatin fertility sperm sperm structure test










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Many of those couples who have been previously unable to conceive with what would be considered extreme measures have been diagnosed with high sperm DNA fragmentation and treated. More importantly, what has also been demonstrated is that the degree of DNA fragmentation correlates very highly with the inability of the sperm to initiate a birth regardless of the technology used to fertilize the egg such as insemination, IVF or ICSI. It is akin to seeing the tip of the iceberg. It is a completely independent variable.

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Dna Fragmentation In Spermatozoa

The sperm chromatin structure assay SCSA is a new diagnostic tool that can detect sperm samples that have a high degree of DNA fragmentation small breaks in the sperm chromosomes. Simply put, DNA is arranged in a double helix or ladder configuration with side rails and rungs. This test uses special stains and complex instrumentation flow cytometer , and a laser beam to quantitate the percentage of sperm with intact vs. The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. It is very important to understand that sperm DNA fragmentation can change with time and it can be improved in many cases. It is now very clear to see that having this information about the quality of the sperm can be tremendously helpful to couples and their physicians.

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What is sperm chromatin structure assay

About a year and a half ago a relatively new concept was introduced to clinical practice. sperm quality was dependent on the amount of damage to the sperm DNA or DNA fragmentation. Those couples using egg donors or surrogates may also benefit from screening prior to going thru the procedures because the effort and costs are so great. Spermatazoa with high DNA fragmentation may fertilize an egg and embryo development stops before implantation or may even initiate a pregnancy, but there is a significantly higher likelihood that it will result in miscarriage. The sperm are then labeled with a special orange colored dye that only attaches to the ends of broken DNA within the sperm cell. The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. This comprehensive and detailed unit presents a rapid, reliable, practical test for DNA integrity based on staining with acridine orange.

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Description: Damaged DNA in the single sperm that fertilizes the female egg can have a dramatic negative impact on fetal development. The integrity of mammalian sperm DNA is of prime importance for the paternal genetic contribution to normal offspring. Several studies had implied that the conventional sperm parameters count, motility and morphology as measured on a routine semen analysis had no bearing on success when ICSI was used. All men with an abnormal semen analysis are candidates for this test as well as men with normal semen analyses who have failed IVF for unexplained reasons. The sperm are then labeled with a special orange colored dye that only attaches to the ends of broken DNA within the sperm cell. The SCSA may be recommended for certain patients, depending on their reproductive history. What has recently been shown in several studies is very interesting and in some ways unexpected. More importantly, what has also been demonstrated is that the degree of DNA fragmentation correlates very highly with the inability of the sperm to initiate a birth regardless of the technology used to fertilize the egg such as insemination, IVF or ICSI. Sperm DNA fragmentation has little or nothing to do with the parameters that we measure on the routine semen analysis.
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